Patent Blue V For Biological Stain

Patent Blue V For Biological Stain

Patent Blue V, also called Food Blue 5, Sulphan Blue, Acid Blue 3, L-Blau 3, C-Blau 20, Patentblau V, Sky Blue, or C.I. 42051 and is a dark bluish synthetic triphenylmethane dye used as a food coloring.[1] As a food additive, it has E number E131. It is a sodium or calcium salt of [4-(α-(4-diethylaminophenyl)-5-hydroxy- 2,4-disulfophenylmethylidene)-2,5-cyclohexadien-1-ylidene] diethylammonium hydroxide inner salt.
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Product Description 

Patent Blue V 

Patent Blue V, also called Food Blue 5, Sulphan Blue, Acid Blue 3, L-Blau 3, C-Blau 20, Patentblau V, Sky Blue, or C.I. 42051 and is a dark bluish synthetic triphenylmethane dye used as a food coloring.[1] As a food additive, it has E number E131. It is a sodium or calcium salt of [4-(α-(4-diethylaminophenyl)-5-hydroxy- 2,4-disulfophenylmethylidene)-2,5-cyclohexadien-1-ylidene] diethylammonium hydroxide inner salt. 

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It is not widely used, but in Europe it can be found in Scotch eggs, certain jelly sweets, blue Curaçao, certain jello varieties
(though not in actual Jell-O brand products), among others. An important advantage is the very deep color it produces even at low concentration, a disadvantage is that it fades fairly quickly when exposed to light.
In medicine, Patent Blue V is used in lymphangiography and sentinel node biopsy as a dye to color lymph vessels. It is also used in dental disclosing tablets as a stain to show dental plaque on teeth.
The color of the dye is pH-dependent. In aqueous solution, its color will vary from a deep blue in alkaline or weakly acidic
medium to a yellow–orange in stronger acidic conditions. It is useful as a pH indicator for the range 0.8–3.0.The structure is also redox-sensitive, changing from a reduced yellow form to an oxidized red form in solution. The reduction potential of around 0.77 volts is similar to that of other triphenylmethane dyes. It is usable as a reversible redox indicator in some analytical methods.
Because of its pH-dependent color, Patent Blue V was included in chemistry sets from Salter Science in the 1970s and 80s under the name Sky Blue.

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